Mohl's half leaf experiment
Experiment to demonstrate Moll’s half-leaf experiment for showing that CO 2 , light, chlorophyll and water are necessary requirements for photosynthesis: Requirements: A potted plant, caustic potash, wide- mouthed bottle, iodine, split cork, water Method: 1. De-starch a potted plant by putting it in complete darkness for two days. 2. Fill partly a wide-mouthed bottle with strong solution of caustic potash and fit a split cork on its mouth. 3. Insert about half of the portion of a leaf of the de-starched plant into the bottle through the split cork (Fig. 36). 4. Place the whole apparatus in light after applying grease on the upper portion of split cork, and test the leaf for stach after about 10 hours. Observations: Portions of the leaf inside the bottle as well as in between the split cork show negative test for starch indicating the absence of photosynthesis while the portions outside the split cork show positive test for starch indicating the presence of process of photosynthesis in
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